Coding

Part:BBa_K895001:Design

Designed by: Frank Sargent   Group: iGEM12_Dundee   (2012-09-21)

Salty_Hcp


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 306
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 133
    Illegal BsaI site found at 150
    Illegal SapI site found at 218


Design Notes

No site-directed mutagenesis required in the construction of this part.


Source

PCR product using Salmonella LT2 genomic DNA as template

References

The Salmonella Type VI Secretion Systems, including the structure and function of Hcp, are reviewed here:

Blondel,C.J., Jimenez,C.J., Contreras,I. and Santiviago,C.A. (2009) Comparative genomic analysis uncovers three novel loci encoding type six secretion systems differentially distributed in Salmonella serotypes. BMC Genomics 10:354.