![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K895001:Design
Designed by: Frank Sargent Group: iGEM12_Dundee (2012-09-21)
Salty_Hcp
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 306
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 133
Illegal BsaI site found at 150
Illegal SapI site found at 218
Design Notes
No site-directed mutagenesis required in the construction of this part.
Source
PCR product using Salmonella LT2 genomic DNA as template
References
The Salmonella Type VI Secretion Systems, including the structure and function of Hcp, are reviewed here:
Blondel,C.J., Jimenez,C.J., Contreras,I. and Santiviago,C.A. (2009) Comparative genomic analysis uncovers three novel loci encoding type six secretion systems differentially distributed in Salmonella serotypes. BMC Genomics 10:354.